Feed-free NK Cell Culture – 原能细胞科技集团

Background

➤ Natural killer(NK) cells play an important role in immune surveillance and tumor cells killing and anti-aging.

➤ The low ability of expansion and the low-purity of NK are the limitations of availability of NK cells。

Schedule

Peripheral blood and apheresis products were obtained from healthy donors (n=2) and activated by NK cell activator for 6 days. At day 4 after activated, cytokines and several necessary factors were supplied. After 7 days, cells were expanded with NK expansion medium supplement with IL-2.

The cell growth curve

Expansion dynamics of PBMC samples obtained from 2 healthy donors and cultured ex vivo for 20 days

Phenotype detection

The phenotype of the expanded NK cell and the proportion of NK cells were higher than 60%.

Expression of activation receptor

feeder-free Nk 试剂盒 增殖效果

Cytokines released

细胞因子 激活效果

Cytotoxicity to K562 cell

feeder-free Nk 试剂盒 增殖 效果

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021-68370606

Address: No. 1118 Halei Road, Pudong New Area, Shanghai

  • Beijing Origincell: 400-810-8282
  • Research and Clinical Transformation Cooperation: 021-68370606
  • Automated Medical Equipment: 021-68395577
  • Life Science and Technology Industrial Park: 021-68370606