高纯度无滋养层NK细胞培养技术 – 原能细胞科技集团

Background

➤ Natural killer(NK) cells play an important role in immune surveillance and tumor cells killing and anti-aging.

➤ The low ability of expansion and the low-purity of NK are the limitations of availability of NK cells.

Schedule

Peripheral blood and apheresis products were obtained from healthy donors (n=2) and activated by NK cell activator for 6 days. At day 4 after activated, cytokines and several necessary factors were supplied. After 7 days, cells were expanded with NK expansion medium supplement with IL-2.

The cell growth curve

Expansion dynamics of PBMC samples obtained from 2 healthy donors and cultured ex vivo for 20 days

Phenotype detection

The phenotype of the expanded NK cell and the proportion of NK cells were higher than 60%。

Expression of activation receptor

feeder-free Nk 试剂盒 增殖效果

Cytokines released

细胞因子 激活效果

Cytotoxicity to K562 cell

feeder-free Nk 试剂盒 增殖 效果